Review Article | Open Access

Volume 2025 |Article ID 100005 | https://doi.org/10.1016/j.bidere.2025.100005

Recent advances on protein engineering for improved stability

Jinghao Shi,^1,2,4 Bo Yuan ,^2,3 Hengquan Yang ,^1,4 Zhoutong Sun ^2,3

¹School of Chemistry and Chemical Engineering, Shanxi University, 030006, Taiyuan, China
²Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 West 7th Avenue, Tianjin Airport Economic Area, Tianjin, 300308, China
³Key Laboratory of Engineering Biology for Low-Carbon Manufacturing, Tianjin, 300308, China
⁴Shanxi Key Laboratory of the Green Catalytic Synthesis of Coal-based High Value Chemicals, China

Abstract

Stability is a crucial factor influencing applicability of industrial biocatalysts, and enzymatic operations in partial organic solvents and higher temperatures are preferable since organic solvents may improve the solubility of the substrates, and the reaction rates are often higher at elevated temperatures. This review aims to summarize recent advances for engineering enzymes to meet the industrial needs for solvent and thermostability and offer insights in the advances in methodologies utilizing B-factors, ancestral reconstructions, or machine learning approaches.